TP63 basal cells are indispensable during endoderm differentiation into proximal airway cells on acellular lung scaffolds.

ABSTRACT: The use of decellularized whole-organ scaffolds for bioengineering of organs is a promising avenue to circumvent the shortage of donor organs for transplantation. However, recellularization of acellular scaffolds from multicellular organs like the lung with a variety of different cell types remains a challenge. Multipotent cells could be an ideal cell source for recellularization. Here we investigated the hierarchical differentiation process of multipotent ES-derived endoderm cells into proximal airway epithelial cells on acellular lung scaffolds. The first cells to emerge on the scaffolds were TP63⁺ cells, followed by TP63⁺/KRT5⁺ basal cells, and finally multi-ciliated and secretory airway epithelial cells. TP63⁺/KRT5⁺ basal cells on

SUBMITTER: Bilodeau C 

PROVIDER: S-EPMC7935966 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature