Sexing chickens (Gallus gallus domesticus) with high-resolution melting analysis using feather crude DNA.

ABSTRACT: Identification of sex in broiler chickens allows researchers to reduce the level of variation in an experiment caused by the sex effect. Broiler breeds commonly used in research are no longer feather sexable because of the change in their genetics. Other alternate sexing methods are costly and difficult to apply on a large scale. Therefore, a sexing method is required that is both cost effective and highly sensitive as well as having the ability to offer high throughput genotyping. In this study, high-resolution melting (HRM) analysis was used to detect DNA variations present in the gene chromodomain helicase DNA binding 1 protein (CHD1) on the Z and W chromosomes (CHD1Z and CHD1W, respectively) of chickens. In addition, a simplified DNA extraction protocol, which made use of the basal par

SUBMITTER: England AD 

PROVIDER: S-EPMC7936197 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature