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Impact of dissolved oxygen and loading rate on NH₃ oxidation and N₂ production mechanisms in activated sludge treatment of sewage.

ABSTRACT: Microaerobic activated sludge (MAS) is a one-stage process operated at 0.5-1.0 mg l^-1 dissolved oxygen (DO) aiming at simultaneous nitrification and denitrification. We used molecular techniques and a comprehensive nitrogen (N)-transformation activity test to investigate the dominant NH₃ -oxidizing and N₂ -producing mechanism as well as the dominant ammonia-oxidizing bacteria (AOB) species in sludge samples individually collected from an MAS system and a conventional anoxic/oxic (A/O) system; both systems were operated at a normal loading rate (i.e. 1.0 kg chemical oxygen demand (COD) m^-3 day^-1 and 0.1 kg NH₄ ⁺ -N m^-3 day^-1 ) in our previous studies. The DO levels in both systems (aerobic: conventional A/O system; microaerobic: MAS system) did not affect the dominant NH₃ -oxidizing mechanism or the dominant AOB species. This study further demonstrated the feasibility of a higher loading rate (i.e. 2.30 kg COD m^-3 day^-1 and 0.34 kg NH₄ ⁺ -N m^-3 day^-1 ) with the MAS process during sewage treatment, which achieved a 40% reduction in aeration energy consumption than that obtained in the conventional A/O system. The increase in loading rates in the MAS system did not affect the dominant NH₃ -oxidizing mechanism but did impact the dominant AOB species. Besides, N₂ was predominantly produced by microaerobic denitrification in the MAS system at the two loading rates.

SUBMITTER: Zhang X

PROVIDER: S-EPMC7936313 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Impact of dissolved oxygen and loading rate on NH3 oxidation and N2 production mechanisms in activated sludge treatment of sewage.

Zhang Xueyu X Li Shida S Zheng Shaokui S Duan Shoupeng S

Microbial biotechnology 20200602 2

Microaerobic activated sludge (MAS) is a one-stage process operated at 0.5-1.0 mg l-1 dissolved oxygen (DO) aiming at simultaneous nitrification and denitrification. We used molecular techniques and a comprehensive nitrogen (N)-transformation activity test to investigate the dominant NH3 -oxidizing and N2 -producing mechanism as well as the dominant ammonia-oxidizing bacteria (AOB) species in sludge samples individually collected from an MAS system and a conventi ...[more]

PMID: 32488999

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Project description:This is the first study that examines the effect of operating conditions on fouling of Membrane Bio-Reactors (MBRs), which treat municipal wastewater in field conditions, with specific regard to the controlled development of filamentous microorganisms (or filaments). The novelty of the present work is extended to minimize the dissolved oxygen (DO) in recirculated activated sludge for improving the process of denitrification. For this purpose, two pilot-scale MBRs were constructed and operated in parallel: i) Filament-MBR, where an attempt was made to regulate the growth of filaments by adjustment of DO, the Food-to-Microorganisms (F/M) ratio and temperature, and ii) Control-MBR, where a gentle stirring tank was employed for the purpose of zeroing the DO in the recycled sludge. Results showed that low temperature (< 15 °C) slightly increased the number of filaments in the Filament-MBR which, in turn, decreased the Trans-Membrane Pressure (TMP). As the Soluble Microbial Products (SMP) and the colloids are considered to be the basic foulants of membranes in MBR systems, specific attention was directed to keep their concentration at low values in the mixed liquor. The low F/M ratio in the aeration tanks which preceded the membrane tank was achieved to keep the SMP proteins and carbohydrates at very low values in the mixed liquor, i.e., less than 6 mg/L. Moreover, as a result of the low recirculation rate (2.6∙Qin), good aggregation of the produced excess sludge was achieved, and low concentration of colloids with a size ≤50 nm (nearly the membranes' pore size used for filtration/separation) was measured, accounted for maximum 15% of the total colloids. Additionally, the increase in filamentous population at the Filament-MBR contributed to the further reduction of colloids in the mixed liquor at 7.9%, contributing beneficially to the reduction of TMP and of membrane fouling. The diminishing of DO in the recirculated sludge improved denitrification, and resulted in lower concentrations of Ν-NO3- and TN in the effluent of the Control-MBR. Furthermore, the recirculation rate of Qr = 2.6∙Qin, in comparison with Qr = 4.3∙Qin, resulted in improved performance regarding the removal of N-NH4+. Finally, high organics removal and ammonium nitrification was observed in the effluent of both pilots, since COD and Ν-ΝΗ4+ concentrations were generally in the range of 10-25 mg/L and < 0.1 mg/L, respectively.

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Dataset Information

Impact of dissolved oxygen and loading rate on NH₃ oxidation and N₂ production mechanisms in activated sludge treatment of sewage.

Publications

Impact of dissolved oxygen and loading rate on NH<sub>3</sub> oxidation and N<sub>2</sub> production mechanisms in activated sludge treatment of sewage.

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