Establishment of a Cuscuta campestris-mediated enrichment system for genomic and transcriptomic analyses of 'Candidatus Liberibacter asiaticus'.

ABSTRACT: 'Candidatus Liberibacter asiaticus' (CLas) is a phloem-limited non-culturable α-proteobacterium associated with citrus Huanglongbing, a highly destructive disease threatening global citrus industry. Research on CLas is challenging due to the current inability to culture CLas in vitro and the low CLas titre in citrus plant. Here, we develop a CLas enrichment system using the holoparasitic dodder plant (Cuscuta campestris) as an amenable host to acquire and enrich CLas from CLas-infected citrus shoots maintained hydroponically. Forty-eight out of fifty-five (87%) dodder plants successfully parasitized CLas-infected citrus shoots with detectable CLas by PCR. Among 48 dodders cultures, 30 showed two- to 419-fold CLas titre increase as compared to the corresponding citrus hosts. The CLas popula

SUBMITTER: Li T 

PROVIDER: S-EPMC7936317 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature