Real-time measurements of aminoglycoside effects on protein synthesis in live cells.

ABSTRACT: The spread of antibiotic resistance is turning many of the currently used antibiotics less effective against common infections. To address this public health challenge, it is critical to enhance our understanding of the mechanisms of action of these compounds. Aminoglycoside drugs bind the bacterial ribosome, and decades of results from in vitro biochemical and structural approaches suggest that these drugs disrupt protein synthesis by inhibiting the ribosome's translocation on the messenger RNA, as well as by inducing miscoding errors. So far, however, we have sparse information about the dynamic effects of these compounds on protein synthesis inside the cell. In the present study, we measured the effect of the aminoglycosides apramycin, gentamicin, and paromomycin on ongoing protein synt

SUBMITTER: Aguirre Rivera J 

PROVIDER: S-EPMC7936356 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature