The CAD risk locus 9p21 increases the risk of vascular calcification in an iPSC-derived VSMC model.

ABSTRACT:

Background

Coronary artery disease (CAD) is the leading cause of death worldwide. Chromosome locus 9p21 was the first to be associated with increased risk of CAD and coronary artery calcification (CAC). Vascular calcification increases the risk for CAD. Vascular smooth muscle cells (VSMCs) are one of the major cell types involved in the development of vascular calcification.

Methods

So far, mainly animal models or primary SMCs have been used to model human vascular calcification. In this study, a human in vitro assay using iPSC-derived VSMCs was developed to examine vascular calcification. Human iPSCs were derived from a healthy non-risk (NR) and risk (R) donor carrying SNPs in the 9p21 locus. Additionally, 9p21 locus knockouts of each donor iPSC line (NR and R) were used. F

SUBMITTER: Trillhaase A 

PROVIDER: S-EPMC7936418 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature