METTL3-mediated m⁶A mRNA modification of FBXW7 suppresses lung adenocarcinoma.

ABSTRACT:

Background

FBXW7 m⁶A modification plays an important role in lung adenocarcinoma (LUAD) progression; however, the underlying mechanisms remain unclear.

Methods

The correlation between FBXW7 and various genes related to m⁶A modification was analyzed using The Cancer Genome Atlas database. The regulatory effects of METTL3 on FBXW7 mRNA m⁶A modification were examined in a cell model, and the underlying mechanism was determined by methylated RNA immunoprecipitation, RNA immunoprecipitation, luciferase reporter, and mutagenesis assays. In vitro experiments were performed to further explore the biological effects of METTL3-mediated FBXW7 m⁶A modification on LUAD development.

Results

Decreased FBXW7 expression was accompanied by do

SUBMITTER: Wu Y 

PROVIDER: S-EPMC7936500 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature