Evaluation of female Aedes aegypti proteome via LC-ESI-MS/MS using two protein extraction methods.

ABSTRACT:

Background

Proteomic analyses have broadened the horizons of vector control measures by identifying proteins associated with different biological and physiological processes and give further insight into the mosquitoes' biology, mechanism of insecticide resistance and pathogens-mosquitoes interaction. Female Ae. aegypti ingests human blood to acquire the requisite nutrients to make eggs. During blood ingestion, female mosquitoes transmit different pathogens. Therefore, this study aimed to determine the best protein extraction method for mass spectrometry analysis which will allow a better proteome profiling for female mosquitoes.

Methods

In this present study, two protein extractions methods were performed to analyze female Ae. aegyti proteome, via TCA acetone

SUBMITTER: Shettima A 

PROVIDER: S-EPMC7936558 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature