A comprehensive evaluation of binning methods to recover human gut microbial species from a non-redundant reference gene catalog.

ABSTRACT: The human gut microbiota performs functions that are essential for the maintenance of the host physiology. However, characterizing the functioning of microbial communities in relation to the host remains challenging in reference-based metagenomic analyses. Indeed, as taxonomic and functional analyses are performed independently, the link between genes and species remains unclear. Although a first set of species-level bins was built by clustering co-abundant genes, no reference bin set is established on the most used gut microbiota catalog, the Integrated Gene Catalog (IGC). With the aim to identify the best suitable method to group the IGC genes, we benchmarked nine taxonomy-independent binners implementing abundance-based, hybrid and integrative approaches. To this purpose, we designed a

SUBMITTER: Borderes M 

PROVIDER: S-EPMC7936653 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature