Identification of unknown acid-resistant genes of oral microbiotas in patients with dental caries using metagenomics analysis.

ABSTRACT: Acid resistance is critical for the survival of bacteria in the dental caries oral micro-environment. However, there are few acid-resistant genes of microbiomes obtained through traditional molecular biology experimental techniques. This study aims to try macrogenomics technologies to efficiently identify acid-resistant genes in oral microbes of patients with dental caries. Total DNA was extracted from oral microbiota obtained from thirty dental caries patients and subjected to high-throughput sequencing. This data was used to build a metagenomic library, which was compared to the sequences of two Streptococcus mutant known acid-resistant genes, danK and uvrA, using a BLAST search. A total of 19 and 35 unknown gene sequences showed similarities with S. mutans uvrA and dnaK in the metagenom

SUBMITTER: Cheng X 

PROVIDER: S-EPMC7936999 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature