Dendritic cell integrin expression patterns regulate inflammation in the rheumatoid arthritis joint.

ABSTRACT:

Objectives

Immune dysregulation contributes to the development of RA. Altered surface expression patterns of integrin adhesion receptors by immune cells is one mechanism by which this may occur. We investigated the role of β2 integrin subunits CD11a and CD11b in dendritic cell (DC) subsets of RA patients.

Methods

Total β2 integrin subunit expression and its conformation ('active' vs 'inactive' state) were quantified in DC subsets from peripheral blood (PB) and SF of RA patients as well as PB from healthy controls. Ex vivo stimulation of PB DC subsets and in vitro-generated mature and tolerogenic monocyte-derived DCs (moDCs) were utilized to model the clinical findings. Integrin subunit contribution to DC function was tested by analysing clustering and adhesion, and in co-cul

SUBMITTER: Schittenhelm L 

PROVIDER: S-EPMC7937020 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature