Exploration of binary protein-protein interactions between tick-borne flaviviruses and Ixodes ricinus.

ABSTRACT:

Background

Louping ill virus (LIV) and tick-borne encephalitis virus (TBEV) are tick-borne flaviviruses that are both transmitted by the major European tick, Ixodes ricinus. Despite the importance of I. ricinus as an arthropod vector, its capacity to acquire and subsequently transmit viruses, known as vector competence, is poorly understood. At the molecular scale, vector competence is governed in part by binary interactions established between viral and cellular proteins within infected tick cells.

Methods

To investigate virus-vector protein-protein interactions (PPIs), the entire set of open reading frames for LIV and TBEV was screened against an I. ricinus cDNA library established from three embryonic tick cell lines using yeast two-hybrid methodology (Y2H). PPIs revealed

SUBMITTER: Lemasson M 

PROVIDER: S-EPMC7937244 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature