Differential gene expression in a tripartite interaction: Drosophila, Spiroplasma and parasitic wasps.

ABSTRACT:

Background

Several facultative bacterial symbionts of insects protect their hosts against natural enemies. Spiroplasma poulsonii strain sMel (hereafter Spiroplasma), a male-killing heritable symbiont of Drosophila melanogaster, confers protection against some species of parasitic wasps. Several lines of evidence suggest that Spiroplasma-encoded ribosome inactivating proteins (RIPs) are involved in the protection mechanism, but the potential contribution of the fly-encoded functions (e.g., immune response), has not been deeply explored.

Methods

Here we used RNA-seq to evaluate the response of D. melanogaster to infection by Spiroplasma and parasitism by the Spiroplasma-susceptible wasp Leptopilina heterotoma, and

SUBMITTER: Higareda Alvear VM 

PROVIDER: S-EPMC7937342 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature