Sputum Processing Method for Lateral Flow Immunochromatographic Assays to Detect Coronaviruses.

ABSTRACT: Coronavirus causes an infectious disease in various species and crosses the species barriers leading to the outbreak of zoonotic diseases. Due to the respiratory diseases are mainly caused in humans and viruses are replicated and excreted through the respiratory tract, the nasal fluid and sputum are mainly used for diagnosis. Early diagnosis of coronavirus plays an important role in preventing its spread and is essential for quarantine policies. For rapid decision and prompt triage of infected host, the immunochromatographic assay (ICA) has been widely used for point of care testing. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, i

SUBMITTER: Kang A 

PROVIDER: S-EPMC7937507 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature