Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene Editing.

ABSTRACT: The CRISPR-Cas system holds great promise in the treatment of diseases caused by genetic variations. The Cas protein, an RNA-guided programmable nuclease, generates a double-strand break at precise genomic loci. However, the use of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas system to distinguish between single-nucleotide variations is challenging. The promiscuity of the guide RNA (gRNA) and its mismatch tolerance make allele-specific targeting an elusive goal. This review presents a meta-analysis of previous studies reporting position-dependent mismatch tolerance within the gRNA. We also examine the conservativity of the seed sequence, a region within the gRNA with stringent sequence dependency, and propose the existence of a subregion within the seed seque

SUBMITTER: Rabinowitz R 

PROVIDER: S-EPMC7938333 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature