Tolerance of Yarrowia lipolytica to inhibitors commonly found in lignocellulosic hydrolysates.

ABSTRACT:

Background

Lignocellulosic material is a suitable renewable carbon and energy source for microbial cell factories, such as Yarrowia lipolytica. To be accessible for microorganisms, the constituent sugars need to be released in a hydrolysis step, which as a side effect leads to the formation of various inhibitory compounds. However, the effects of these inhibitory compounds on the growth of Y. lipolytica have not been thoroughly investigated.

Results

Here we show the individual and combined effect of six inhibitors from three major inhibitor groups on the growth of Y. lipolytica. We engineered a xylose consuming strain by overexpressing the three native genes XR, XDH, and XK and found that the inhibitor tolerance of Y. lipolytica is similar in glucose and in xylose. Aromatic

SUBMITTER: Konzock O 

PROVIDER: S-EPMC7938539 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature