Construction of a bivalent vaccine against anthrax and smallpox using the attenuated vaccinia virus KVAC103.

ABSTRACT:

Background

Anthrax and smallpox are high-risk infectious diseases, and considered as potential agents for bioterrorism. To develop an effective countermeasure for these diseases, we constructed a bivalent vaccine against both anthrax and smallpox by integrating a gene encoding protective antigen (PA) of Bacillus anthracis to the genome of the attenuated vaccinia virus strain, KVAC103.

Results

Immunization with this bivalent vaccine induced antibodies against both PA and vaccinia virus in a mouse model. We also observed that the efficacy of this vaccine can be enhanced by combined immunization with immunoadjuvant-expressing KVAC103. Mouse groups co-immunized with PA-expressing KVAC103 and either interleukin-15 (IL-15) or cholera toxin subunit A (CTA1)-expressing KVAC103 showe

SUBMITTER: Park DB 

PROVIDER: S-EPMC7938549 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature