Obesity-associated inflammation induces androgenic to estrogenic switch in the prostate gland.

ABSTRACT:

Background and objective

Our patient cohort revealed that obesity is strongly associated with steroid-5α reductase type 2 (SRD5A2) promoter methylation and reduced protein expression. The underlying mechanism of prostatic growth in this population is poorly understood. Here we addressed the question of how obesity, inflammation, and steroid hormones affect the development of benign prostatic hyperplasia (BPH).

Material and methods

We used preadipocytes, macrophages, primary human prostatic stromal cells, prostate tissues from high-fat diet-induced obese mice, and 35 prostate specimens that were collected from patients who underwent transurethral resection of the prostate (TURP). RNA was isolated and quantified with RT-PCR. Genome DNA was extracted and SRD5A2 promoter methyla

SUBMITTER: Xue B 

PROVIDER: S-EPMC7938647 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature