BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

ABSTRACT: Connectivity webs mediate the unique biology of the mammalian brain. Yet, while cell circuit maps are increasingly available, knowledge of their underlying molecular networks remains limited. Here, we applied multi-dimensional biochemical fractionation with mass spectrometry and machine learning to survey endogenous macromolecules across the adult mouse brain. We defined a global "interactome" comprising over one thousand multi-protein complexes. These include hundreds of brain-selective assemblies that have distinct physical and functional attributes, show regional and cell-type specificity, and have links to core neurological processes and disorders. Using reciprocal pull-downs and a transgenic model, we validated a putative 28-member RNA-binding protein complex associated with amyotroph

SUBMITTER: Pourhaghighi R 

PROVIDER: S-EPMC7938770 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature