Defining Endothelial Cell-Derived Factors That Promote Pericyte Recruitment and Capillary Network Assembly.

ABSTRACT:

Objective

We sought to identify and investigate the functional role of the major endothelial cell (EC)-derived factors that control pericyte recruitment to EC tubes and pericyte-induced tube maturation during capillary network formation. Approach and Results: We identify PDGF (platelet-derived growth factor)-BB, PDGF-DD, ET (endothelin)-1, TGF (transforming growth factor)-β, and HB-EGF (heparin-binding epidermal growth factor), as the key individual and combined regulators of pericyte assembly around EC tubes. Using novel pericyte only assays, we demonstrate that PDGF-BB, PDGF-DD, and ET-1 are the primary direct drivers of pericyte invasion. Their addition to pericytes induces invasion as if ECs were present. In contrast, TGF-β and HB-EGF have minimal ability to directly stimulate

SUBMITTER: Kemp SS 

PROVIDER: S-EPMC7939110 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature