De novo identification and targeted sequencing of SSRs efficiently fingerprints Sorghum bicolor sub-population identity.

ABSTRACT: Recent plant breeding studies of several species have demonstrated the utility of combining molecular assessments of genetic distance into trait-linked SNP genotyping during the development of parent lines to maximize yield gains due to heterosis. SSRs (Short Sequence Repeats) are the molecular marker of choice to determine genetic diversity, but the methods historically used to sequence them have been burdensome. The ability to analyze SSRs in a higher-throughput manner independent of laboratory conditions would increase their utility in molecular ecology, germplasm curation, and plant breeding programs worldwide. This project reports simple bioinformatics methods that can be used to generate genome-wide de novo SSRs in silico followed by targeted Next Generation Sequencing (NGS) validati

SUBMITTER: Baggett JP 

PROVIDER: S-EPMC7939377 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature