Circ-UBAP2 functions as sponges of miR-1205 and miR-382 to promote glioma progression by modulating STC1 expression.

ABSTRACT:

Background

Circular RNAs (circRNAs) exert vital functions in glioma pathogenesis. CircRNA ubiquitin-associated protein 2 (circ-UBAP2, hsa_circ_0008344) has been illuminated as a tumor driver in glioma. Nevertheless, the mechanisms underlying the oncogenic regulation of circ-UBAP2 in glioma are still undefined.

Methods

Circ-UBAP2, miR-1205, miR-382, and GPRC5A were quantified using qRT-PCR and western blot. Cell viability was detected using a CCK-8 assay. Cell migration and invasion were measured using the would-healing and transwell assays. Flow cytometry and colony formation assay were applied to evaluate cell apoptosis and colony formation, respectively. The xenograft model assays were used to examine the impact of circ-UBAP2 on tumorigenic effect in vivo. Direct relations

SUBMITTER: Wang J 

PROVIDER: S-EPMC7940226 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature