Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis.

ABSTRACT: 3-Hydroxypropionic acid (3HP), an important three carbon (C3) chemical, is designated as one of the top platform chemicals with an urgent need for improved industrial production. Halomonas bluephagenesis shows the potential as a chassis for competitive bioproduction of various chemicals due to its ability to grow under an open, unsterile and continuous process. Here, we report the strategy for producing 3HP and its copolymer poly(3-hydroxybutyrate-co-3-hydroxypropionate) (P3HB3HP) by the development of H. bluephagenesis. The transcriptome analysis reveals its 3HP degradation and synthesis pathways involving endogenous synthetic enzymes from 1,3-propanediol. Combing the optimized expression of aldehyde dehydrogenase (AldD_Hb), an engineered H. bluephagenesis strain of whose 3HP de

SUBMITTER: Jiang XR 

PROVIDER: S-EPMC7940609 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature