A hepatitis B virus transgenic mouse model with a conditional, recombinant, episomal genome.

ABSTRACT:

Background & aims

Development of new and more effective therapies against hepatitis B virus (HBV) is limited by the lack of suitable small animal models. The HBV transgenic mouse model containing an integrated overlength 1.3-mer construct has yielded crucial insights, but this model unfortunately lacks covalently closed circular DNA (cccDNA), the episomal HBV transcriptional template, and cannot be cured given that HBV is integrated in every cell.

Methods

To solve these 2 problems, we generated a novel transgenic mouse (HBV1.1X), which generates an excisable circular HBV genome using Cre/LoxP technology. This model possesses a HBV1.1-mer cassette knocked into the ROSA26 locus and is designed for stable expression of viral proteins from birth, like the current HBV tran

SUBMITTER: Kruse RL 

PROVIDER: S-EPMC7940981 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature