CTGF facilitates cell-cell communication in chondrocytes via PI3K/Akt signalling pathway.

ABSTRACT:

Purposes

Gap junction intercellular communication (GJIC) is essential for articular cartilage to respond appropriately to physical or biological stimuli and maintain homeostasis. Connective tissue growth factor (CTGF), identified as an endochondral ossification genetic factor, plays a vital role in cell proliferation, migration and adhesion. However, how CTGF regulates GJIC in chondrocytes is still unknown. This study aims to explore the effects of CTGF on GJIC in chondrocytes and its potential biomechanism.

Materials and methods

qPCR was performed to determine the expression of gene profile in the CCN family in chondrocytes. After CTGF treatment, CCK-8 assay and scratch assay were performed to explore cell proliferation and migration. A scrape loading/dye transfer assay was

SUBMITTER: Wu Z 

PROVIDER: S-EPMC7941231 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature