Circ_SMAD4 promotes gastric carcinogenesis by activating wnt/β-catenin pathway.

ABSTRACT:

Objectives

Circular RNAs (circRNAs) are essential participants in tumour progression. This study focused on investigating the mechanism of a novel functional circRNA in gastric cancer (GC).

Methods

Gene expression was detected by qRT-PCR or Western blot. Survival curves were generated via Kaplan-Meier method. In vitro and in vivo assays were used to investigate the impact of circ_SMAD4 on GC cell growth and tumorigenesis. Agarose gel electrophoresis assay, RNase R treatment and Sanger sequencing were utilized for confirming the circular structure of circ_SMAD4. Relationship between molecules was monitored by a series of mechanical experiments, as needed.

Results

Circ_SMAD4 expression was potentiated in GC. Circ_SMAD4 depletion impeded GC cell growth in vitro and restr

SUBMITTER: Wang L 

PROVIDER: S-EPMC7941240 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature