High-pressure small-angle X-ray scattering cell for biological solutions and soft materials.

ABSTRACT: Pressure is a fundamental thermodynamic parameter controlling the behavior of biological macromolecules. Pressure affects protein denaturation, kinetic parameters of enzymes, ligand binding, membrane permeability, ion trans-duction, expression of genetic information, viral infectivity, protein association and aggregation, and chemical processes. In many cases pressure alters the molecular shape. Small-angle X-ray scattering (SAXS) is a primary method to determine the shape and size of macromolecules. However, relatively few SAXS cells described in the literature are suitable for use at high pressures and with biological materials. Described here is a novel high-pressure SAXS sample cell that is suitable for general facility use by prioritization of ease of sample loading, temperature contr

SUBMITTER: Rai DK 

PROVIDER: S-EPMC7941318 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature