Tumorigenic effects of TLX overexpression in HEK 293T cells.

ABSTRACT:

Background

The human orphan receptor TLX (NR2E1) is a key regulator of neurogenesis, adult stem cell maintenance, and tumorigenesis. However, little is known about the genetic and transcriptomic events that occur following TLX overexpression in human cell lines.

Aims

Here, we used cytogenetics and RNA sequencing to investigate the effect of TLX overexpression with an inducible vector system in the HEK 293T cell line.

Methods and results

Conventional spectral karyotyping was used to identify chromosomal abnormalities, followed by fluorescence in situ hybridization (FISH) analysis on chromosome spreads to assess TLX DNA copy number. Illumina paired-end whole transcriptome sequencing was then performed to characterize recurrent genetic variants (single nucleotide polymor

SUBMITTER: Parris TZ 

PROVIDER: S-EPMC7941458 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature