RhoB is regulated by hypoxia and modulates metastasis in breast cancer.

ABSTRACT:

Background

RhoB is a Rho family GTPase that is highly homologous to RhoA and RhoC. RhoA and RhoC have been shown to promote tumor progression in many cancer types; however, a distinct role for RhoB in cancer has not been delineated. Additionally, several well-characterized studies have shown that small GTPases such as RhoA, Rac1, and Cdc42 are induced in vitro under hypoxia, but whether and how hypoxia regulates RhoB in breast cancer remains elusive.

Aims

To determine whether and how hypoxia regulates RhoB expression and to understand the role of RhoB in breast cancer metastasis.

Methods

We investigated the effects of hypoxia on the expression and activation of RhoB using real-time quantitative polymerase chain reaction and western blotting. We also examined the signi

SUBMITTER: Ju JA 

PROVIDER: S-EPMC7941481 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature