Vaccination with SARS-CoV-2 Spike Protein and AS03 Adjuvant Induces Rapid Anamnestic Antibodies in the Lung and Protects Against Virus Challenge in Nonhuman Primates.

ABSTRACT: Adjuvanted soluble protein vaccines have been used extensively in humans for protection against various viral infections based on their robust induction of antibody responses. Here, soluble prefusion-stabilized spike trimers (preS dTM) from the severe acute respiratory syndrome coronavirus (SARS-CoV-2) were formulated with the adjuvant AS03 and administered twice to nonhuman primates (NHP). Binding and functional neutralization assays and systems serology revealed that NHP developed AS03-dependent multi-functional humoral responses that targeted multiple spike domains and bound to a variety of antibody F_C receptors mediating effector functions in vitro. Pseudovirus and live virus neutralizing IC₅₀ titers were on average greater than 1000 and significantly highe

SUBMITTER: Francica JR 

PROVIDER: S-EPMC7941623 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature