C11orf95-RELA fusion drives aberrant gene expression through the unique epigenetic regulation for ependymoma formation.

ABSTRACT: Recurrent C11orf95-RELA fusions (RELA^FUS) are the hallmark of supratentorial ependymomas. The presence of RELA as the fusion partner indicates a close association of aberrant NF-κB activity with tumorigenesis. However, the oncogenic role of the C11orf95 has not been determined. Here, we performed ChIP-seq analyses to explore genomic regions bound by RELA^FUS and H3K27ac proteins in human 293T and mouse ependymoma cells. We then utilized published RNA-Seq data from human and mouse RELA^FUS tumors and identified target genes that were directly regulated by RELA^FUS in these tumors. Subsequent transcription factor motif analyses of RELA^FUS target genes detected a unique GC-rich motif recognized by the C11orf95 moiety, that is present in appr

SUBMITTER: Ozawa T 

PROVIDER: S-EPMC7941712 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature