Pi-starvation induced transcriptional changes in barley revealed by a comprehensive RNA-Seq and degradome analyses

ABSTRACT:

Background

Small RNAs (sRNAs) are 20–30?nt regulatory elements which are responsible for plant development regulation and participate in many plant stress responses. Insufficient inorganic phosphate (Pi) concentration triggers plant responses to balance the internal Pi level.

Results

In this study, we describe Pi-starvation-responsive small RNAs and transcriptome changes in barley (Hordeum vulgare L.) using Next-Generation Sequencing (NGS) RNA-Seq data derived from three different types of NGS libraries: (i) small RNAs, (ii) degraded RNAs, and (iii) functional mRNAs. We find that differentially and significantly expressed miRNAs (DEMs, Bonferroni adjusted p-value Conclusions Our results provide comprehensive data to demonstrate complex responses at the RNA level in barley to maintain Pi homeostasis and indicate that barley adapts to Pi-starvation through elicitation of RNA degradation. Novel P-responsive genes were selected as putative candidates to overcome low-Pi stress in barley plants.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12864-021-07481-w.

SUBMITTER: Sega P 

PROVIDER: S-EPMC7941915 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature