Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae.

ABSTRACT:

Background

Saccharomyces cerevisiae is a well-known popular model system for basic biological studies and serves as a host organism for the heterologous production of commercially interesting small molecules and proteins. The central metabolism is at the core to provide building blocks and energy to support growth and survival in normal situations as well as during exogenous stresses and forced heterologous protein production. Here, we present a comprehensive study of intracellular central metabolite pool profiling when growing S. cerevisiae on different carbon sources in batch cultivations and at different growth rates in nutrient-limited glucose chemostats. The latest versions of absolute quantitative mass spectrometry-based metabolite profiling methodology were applied to cover

SUBMITTER: Kumar K 

PROVIDER: S-EPMC7941957 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature