Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4.

ABSTRACT:

Background

Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions without more sophisticated equipment.

Method

The rapid reverse transcription recombinase-aided amplification (RT-RAA) is a fast, robust and isothermal nucleic acid detection method based on enzyme reaction. This method can complete the sample detection at 39 °C in 30 min. In this study, we successfully established a rapid reverse transcription recombinase-aided amplification (RT-RAA) assay for the detection of human norovirus GII.4 and applied this assay to clinical samples, as well as comparison with commercial reverse tran

SUBMITTER: Qin Z 

PROVIDER: S-EPMC7941963 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature