Proteomics-based approach for differentiation of age-related macular degeneration sub-types.

ABSTRACT:

Purpose

Age-related macular degeneration (AMD) is one of the leading causes of irreversible central vision loss in the elderly population. The current study aims to find non-invasive prognostic biomarkers in the urine specimens of the AMD patients.

Methods

Peripheral blood and urine samples were collected from 23 controls and 61 AMD patients. Genomic DNA was extracted from the buffy coat of peripheral blood. Allele specific PCR was used to assay SNPs in complement factor H (CFH), complement component 3 (C3). Comparative proteomic analysis of urine samples from early AMD, choroidal neovascular membrane (CNVM), geographic atrophy (GA), and healthy controls was performed using isobaric labelling followed by mass spectrometry. Validation was performed using enzyme-linked immunos

SUBMITTER: Sivagurunathan S 

PROVIDER: S-EPMC7942106 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature