Selective inhibition of V600E-mutant BRAF gene induces apoptosis in thyroid carcinoma cell lines.

ABSTRACT:

Purpose

Papillary thyroid cancer (PTC) has a high incidence of BRAF^V600E mutation. The purpose of this study was to evaluate the potential relationship between thyroiditis and BRAF^V600E mutation status in patients with PTC. We investigated how a selective inhibitor of BRAF^V600E PLX4032 affects the proliferation and inflammatory cytokine levels of thyroid cancer.

Methods

Two thyroid cancer cell lines TPC1 and 8505C were treated with PLX4032, an analysis was done on cell growth, cell cycle, the degree of apoptosis, and levels of inflammatory cytokines. To identify the functional links of BRAF, we used the STRING database.

Results

Docking results illustrated PLX4032 blocked the kinase activity by exclusively binding on the serine/threonin

SUBMITTER: Park KS 

PROVIDER: S-EPMC7943282 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature