Inhibition of miR-200b Promotes Angiogenesis in Endothelial Cells by Activating The Notch Pathway.

ABSTRACT:

Objective

Patients with diabetes mellitus frequently have chronic wounds or diabetic ulcers as a result of impaired wound healing, which may lead to limb amputation. Human umbilical vein endothelial cell (HUVEC) dysfunction also delays wound healing. Here, we investigated the mechanism of miR-200b in HUVECs under high glucose conditions and the potential of miR-200b as a therapeutic target.

Materials and methods

In this experimental study, HUVECs were cultured with 5 or 30 mM glucose for 48 hours. Cell proliferation was evaluated by CCK-8 assays. Cell mobility was tested by wound healing and Transwell assays. Angiogenesis was analyzed in vitro Matrigel tube formation assays. Luciferase reporter assays were used to test the binding of miR-200b with Notch1.

Results</

SUBMITTER: Qiu TY 

PROVIDER: S-EPMC7944128 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature