Study on the differential proteomics of rat hippocampal mitochondria during deep hypothermic circulatory arrest.

ABSTRACT:

Background

This study aimed to investigate the effect of deep hypothermic circulatory arrest (DHCA) on rat hippocampal mitochondrial protein expression and its differential proteomics, and explore the potential mechanisms behind the effect.

Methods

We used internal jugular vein reflux and tail artery perfusion methods to establish the rat cardiopulmonary bypass (CPB) model. Rats were dissected to obtain the hippocampus, and the hippocampal mitochondria were purified. The mitochondrial morphology and the mitochondrial marker cytochrome C oxidase (COX) qualitatively examined via transmission electron microscopy and western-blot analysis, respectively. The qualified samples were subjected to isobaric tags for relative and absolute quantification (iTRAQ); we then established the

SUBMITTER: Gao Y 

PROVIDER: S-EPMC7944285 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature