Phosphatase Shp2 regulates biogenesis of small extracellular vesicles by dephosphorylating Syntenin.

ABSTRACT: As novel mediators of cell-to-cell signalling, small extracellular vesicles (sEVs) play a critical role in physiological and pathophysiological processes. To date, the molecular mechanisms that support sEV generation are incompletely understood. Many kinases are reported for their roles in sEV generation or composition, whereas the involvement of phosphatases remains largely unexplored. Here we reveal that pharmacological inhibition and shRNA-mediated down-regulation of tyrosine phosphatase Shp2 significantly increases the formation of sEVs. By Co-immunoprecipitation (Co-IP) and in vitro dephosphorylation assays, we identified that Shp2 negatively controlled sEV biogenesis by directly dephosphorylating tyrosine 46 of Syntenin, which has been reported as a molecular switch in sEV biogenesis

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7944561 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature