High dose expression of heme oxigenase-1 induces retinal degeneration through ER stress-related DDIT3.

ABSTRACT:

Background

Oxidative stress is a common cause of neurodegeneration and plays a central role in retinal degenerative diseases. Heme oxygenase-1 (HMOX1) is a redox-regulated enzyme that is induced in neurodegenerative diseases and acts against oxidative stress but can also promote cell death, a phenomenon that is still unexplained in molecular terms. Here, we test whether HMOX1 has opposing effects during retinal degeneration and investigate the molecular mechanisms behind its pro-apoptotic role.

Methods

Basal and induced levels of HMOX1 in retinas are examined during light-induced retinal degeneration in mice. Light damage-independent HMOX1 induction at two different expression levels is achieved by intraocular injection of different doses of an adeno-associated virus vector

SUBMITTER: Li H 

PROVIDER: S-EPMC7944639 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature