Comparative metabolomics analysis of amphotericin B high-yield mechanism for metabolic engineering.

ABSTRACT:

Background

The polyene macrocyclic compound amphotericin B (AmB) is an important antifungal antibiotic for the clinical treatment of invasive fungal infections. To rationally guide the improvement of AmB production in the main producing strain Streptomyces nodosus, comparative metabolomics analysis was performed to investigate the intracellular metabolic changes in wild-type S. nodosus ZJB20140315 with low-yield AmB production and mutant S. nodosus ZJB2016050 with high-yield AmB production, the latter of which reached industrial criteria on a pilot scale.

Results

To investigate the relationship of intracellular metabolites, 7758 metabolites were identified in mutant S. nodosus and wildtype S. nodosus via LC-MS. Through analysis of metabolism, the level of 26 key metabolites

SUBMITTER: Zhang B 

PROVIDER: S-EPMC7945361 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature