TOX3 Promotes Ovarian Estrogen Synthesis: An RNA-Sequencing and Network Study.

ABSTRACT:

Background

Women who undergo chronic exposure to excessive estrogen are at a high risk of developing breast cancer. TOX3 has been reported to be highly expressed in breast tumors and is closely related to estrogen receptors. However, the effect of TOX3 on estrogen synthesis remains poorly understood.

Methods

Using lentiviruses as a vector, we stably overexpressed TOX3 in the ovarian granulosa cell line KGN, the cells where estradiol is primarily produced, to investigate its role in estrogen production as well as cell viability and apoptosis. RNA-Sequencing was applied to uncover the global gene expression upon TOX3 overexpression.

Results

We observed an increased level of cell viability and a reduced cell apoptosis rate after TOX3 overexpression, and the level

SUBMITTER: Man Y 

PROVIDER: S-EPMC7945945 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature