Epigenomic characterization of latent HIV infection identifies latency regulating transcription factors.

ABSTRACT: Transcriptional silencing of HIV in CD4 T cells generates a reservoir of latently infected cells that can reseed infection after interruption of therapy. As such, these cells represent the principal barrier to curing HIV infection, but little is known about their characteristics. To further our understanding of the molecular mechanisms of latency, we characterized a primary cell model of HIV latency in which infected cells adopt heterogeneous transcriptional fates. In this model, we observed that latency is a stable, heritable state that is transmitted through cell division. Using Assay of Transposon-Accessible Chromatin sequencing (ATACseq) we found that latently infected cells exhibit greatly reduced proviral accessibility, indicating the presence of chromatin-based structural barriers t

SUBMITTER: Jefferys SR 

PROVIDER: S-EPMC7946360 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature