Intestinal Lymph Collection via Cannulation of the Mesenteric Lymphatic Duct in Mice.

ABSTRACT:

Background

We have optimized a technique for cannulation of mesenteric lymph duct (MLD) in mice. Mice have low rates of intestinal lymph production; the MLDs are smaller and associated with fragile vasculature. Previous protocols for lymph collection based on the open lymph fistula model were associated with low success rates in mice. Bariatric surgery procedures worsen success rates due to postoperative adhesions and GI rearrangement. We have used this procedure to collect mesenteric lymph from mice undergoing bile diversion from gall bladder to ileum (GB-IL).

Hypothesis

We hypothesize that peptide YY (PYY) levels in mesenteric lymph will increase following nutrient delivery in mice undergoing bile diversion from gall bladder to ileum (GB-IL).

Methods and results

We

SUBMITTER: Banan B 

PROVIDER: S-EPMC7946809 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature