Endophilin recruitment drives membrane curvature generation through coincidence detection of GPCR loop interactions and negative lipid charge.

ABSTRACT: Endophilin plays key roles during endocytosis of cellular receptors, including generating membrane curvature to drive internalization. Electrostatic interactions between endophilin's BIN/Amphiphysin/Rvs domain and anionic membrane lipids have been considered the major driving force in curvature generation. However, the SH3 domain of endophilin also interacts with the proline-rich third intracellular loop (TIL) of various G-protein-coupled receptors (GPCRs), and it is unclear whether this interaction has a direct role in generating membrane curvature during endocytosis. To examine this, we designed model membranes with a membrane density of 1400 receptors per μm² represented by a covalently conjugated TIL region from the β1-adrenergic receptor. We observed that TIL recruits endop

SUBMITTER: Mondal S 

PROVIDER: S-EPMC7948419 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature