Fc γ receptor IIIa/CD16a processing correlates with the expression of glycan-related genes in human natural killer cells.

ABSTRACT: Many therapeutic monoclonal antibodies require binding to Fc γ receptors (FcγRs) for full effect and increasing the binding affinity increases efficacy. Preeminent among the five activating human FcγRs is FcγRIIIa/CD16a expressed by natural killer (NK) cells. CD16a is heavily processed, and recent reports indicate that the composition of the five CD16a asparagine(N)-linked carbohydrates (glycans) impacts affinity. These observations indicate that specific manipulation of CD16a N-glycan composition in CD16a-expressing effector cells including NK cells may improve treatment efficacy. However, it is unclear if modifying the expression of select genes that encode processing enzymes in CD16a-expressing effector cells is sufficient to affect N-glycan composition. We identified substantial proces

SUBMITTER: Patel KR 

PROVIDER: S-EPMC7948478 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature