Cross-seeding between the functional amyloidogenic CRES and CRES3 family members and their regulation of Aβ assembly.

ABSTRACT: Accumulating evidence shows that amyloids perform biological roles. We previously showed that an amyloid matrix composed of four members of the CRES subgroup of reproductive family 2 cystatins is a normal component of the mouse epididymal lumen. The cellular mechanisms that control the assembly of these and other functional amyloid structures, however, remain unclear. We speculated that cross-seeding between CRES members could be a mechanism to control the assembly of the endogenous functional amyloid. Herein we used thioflavin T assays and negative stain transmission electron microscopy to explore this possibility. We show that CRES3 rapidly formed large networks of beaded chains that possessed the characteristic cross-β reflections of amyloid when examined by X-ray diffraction. The beade

SUBMITTER: Do HQ 

PROVIDER: S-EPMC7948811 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature