The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells.

ABSTRACT: BC200 is a noncoding RNA elevated in a broad spectrum of tumor cells that is critical for cell viability, invasion, and migration. Overexpression studies have implicated BC200 and the rodent analog BC1 as negative regulators of translation in both cell-based and in vitro translation assays. Although these studies are consistent, they have not been confirmed in knockdown studies and direct evidence for this function is lacking. Herein, we have demonstrated that BC200 knockdown is correlated with a decrease in global translation rates. As this conflicts with the hypothesis that BC200 is a translational suppressor, we overexpressed BC200 by transfection of in vitro transcribed RNA and transient expression from transfected plasmids. In this context BC200 suppressed translation; however, an inn

SUBMITTER: Booy EP 

PROVIDER: S-EPMC7949042 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature