Pseudomonas aeruginosa detachment from surfaces via a self-made small molecule.

ABSTRACT: Pseudomonas aeruginosa is a significant threat in both healthcare and industrial biofouling. Surface attachment of P. aeruginosa is particularly problematic as surface association induces virulence and is necessary for the ensuing process of biofilm formation, which hampers antibiotic treatments. Previous efforts have searched for dispersal agents of mature biofilm collectives, but there are no known factors that specifically disperse individual surface-attached P. aeruginosa. In this study, we develop a quantitative single-cell surface-dispersal assay and use it to show that P. aeruginosa itself produces factors that can stimulate its dispersal. Through bioactivity-guided fractionation, mass spectrometry, and nuclear magnetic resonance, we elucidated the structure of one such factor, 2-me

SUBMITTER: Scheffler RJ 

PROVIDER: S-EPMC7949062 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature