Calmodulin binds and modulates K⁺-dependent Na⁺/Ca²⁺-exchanger isoform 4, NCKX4.

ABSTRACT: The family of K⁺-dependent Na⁺/Ca²⁺-exchangers, NCKX, are important mediators of cellular Ca²⁺ efflux, particularly in neurons associated with sensory transduction. The NCKX family comprises five proteins, NCKX1-5, each being the product of a different SLC24 gene. NCKX4 (SLC24A4) has been found to have a critical role in termination and adaptation of visual and olfactory signals, melanocortin-dependent satiety signaling, and the maturation of dental enamel. To explore mechanisms that might influence the temporal control of NCKX4 activity, a yeast two-hybrid system was used to search for protein interaction partners. We identified calmodulin as a partner for NCKX4 and confirmed the interaction using glutathione-S-transferase fusion pull-down. Calm

SUBMITTER: Thibodeau S 

PROVIDER: S-EPMC7949085 | biostudies-literature | 2021 Jan-Jun

REPOSITORIES: biostudies-literature